Discover the Phytronix LDTD Source
The LDTD Source of the company Phytronix allows by completely eliminating the stage of liquid chromatography (HPLC / UHPLC) to considerably increase your analytical rates.
The LDTD® source combines an APCI (Atmospheric Pressure Chemical Ionization) chemical ionization with a laser diode high-speed thermodesorption.
A sample can be analyzed every 4 to 10 seconds. You multiply your rates between 20 and 100 X. You can typically analyze more than 1000 samples in less than 2 hours.
The LDTD® source is “Plug & Play” and replaces the installed source initially installed on your device. It is easily interchangeable with the conventional source to easily switch from LDTD / MSMS mode to LC / MSMS mode.
The LDTD® source is for AB SCIEX®, Waters® and Thermo Scientific® AB mass spectrometer users in the Pharmaceutical, Medical, Legal, Environmental, and Agri-Food markets.
The targeted segments / laboratories are in-Vitro trials in the early stages of drug development, toxicology, general bioanalysis, water analysis and routine food and research laboratories.
THE PRINCIPE OF FONCTIONMENT
A constant airflow (in blue) will drive the molecules through the transfer tube to the end where the Corona discharge needle will achieve APCI ionization.
The ions thus formed will then enter the mass spectrometer for analysis and detection.
This entire process takes about 10 seconds.
- 57th ASMS Highly Efficient Ionization of Liquid Crystals by Laser Diode Thermal Desorption-Atmospheric Pressure Chemical Ionization (LDTD-APCI).
EFFECTIVENESS OF APCI IONIZATION IN THE CASA OF LDTD
The introduction of a volume of between 0.5 and 10 μl into a well leads by evaporating the solvent to the formation of a nanoscale crystal layer on the stainless steel sheet forming the bottom of the well. Applying laser radiation to the back of the stainless steel layer for a very short time (typically 4-6 seconds) allows the substance in the form of nano-crystals to thermally desorb into the gas phase. neutral compounds and at a temperature below the melting point. In addition, the newly desorbed molecules enter a gas flow at 3 l / min, which acts as a heat buffer.
Neutrals enter the Corona discharge area to undergo APCI. The main source of protons (APCI +) is provided by the water contained in the compressed air of a bottle or compressor. The research demonstrated the presence of H3O + and (H2O) H3O + ions as the species initiating ionization by a direct proton transfer reaction. Unlike traditional APCI where proton transfer occurs via protonated solvent molecules or water clusters ((H2O) nH3O + where n ≥ 3) the proton transfer involved in the LDTD is very efficient.
ABOUT SAMPLE PREPARATION
The LDTD® source is compatible with all types of extraction procedures such as liquid-liquid extraction, SPE and protein precipitation. Small adjustments may be necessary to perform LDTD analysis such as dilution of the extract, increasing the volume of the organic phase (liq-liq extraction), decreasing the sample size or increasing washing steps in SPE.
The LDTD® source is very robust to the often observed ion suppression phenomena. A highly concentrated solution of salts (10 mg / ml in water) can be analyzed in LDTD without observing ionic suppression. During the thermal desorption phase, the salt remains in the well when the molecules of interest are transferred into the gas phase and subjected to APCI ionization.
MAINTENANCE / SERVICE
The LDTD® source requires only compressed air. No solvent, no tubing to inspect and no leakage. The installation is carried out by us and the equipment is guaranteed 1 year parts, labor and travel.
We also offer qualifications (QI / QO / PQ) upon request.
THE APPLICATIONS OF THE LDTD SOURCE
In this process adsorption, distribution, metabolism, excretion and toxicology (ADMET) must be evaluated within a living organism. Therefore, increasing the flow of analysis for such analyzes is essential.
So far, the “bottleneck” is the LC-MS / MS analysis. Using the LDTD for ADMET studies, you perform a scan every four seconds which greatly increases your productivity compared to your conventional LC-MS / MS method.
In addition, many other applications, such as pharmacokinetic studies, can benefit from this speed of analysis.
Quantification of drugs in the plasma sample can be as fast as 4 seconds per sample without compromising your accuracy.
You want more information to understand how the LDTD source performs your drug quantification … Click here to view the applications.
CONTRACT RESEARCH ORGANIZATION – CRO
Drug testing in urine and plasma using LDTD ionization is 50 times faster than published data.
Rapid screening combined with accurate quantification results will allow your lab to increase your analytical capacity and reduce your sequences.
And now ? Discover the LDTD source used in toxicological analyzes.
Analysis of BTEX in urban air, endocrine disruptors and identification of antibiotics in surface water exposure show how the LDTD source can perform trace analysis in environmental matrices.
Complex matrices such as food are a challenge for all analytical systems. The robustness of the LDTD and its speed are the solutions for this kind of challenge allowing, for example, the analysis of cow’s milk and honey for antibiotic residues without a long preparation of the sample.
NEED A DEMONSTRATION?
We propose two approaches:
ThermoFisher TSQ Vantage triple quadrupole
Waters XEVO TQ MS triple quadrupole
Sciex 5600 Triple TOF
MODELS AND COMPATIBILITY
S-960 / S-3840 for SCIEX® Spectrometers
- API 3000, 4000 and 5000 Triple Quadrupole
- Q TRAP 3200, 4000
- AB SCIEX Q TRAP 5500
- AB SCIEX TripleTOFTM 5600
T-960 / T-3840 for Thermo® Spectrometers
- TSQ Quanttum serie Triple Quadrupole
- TSQ Vantage Triple Quadrupole
- LTQ XL
WX-960 / WX-3840 for Waters® Spectrometers
- Synapt G2 (model HDMS and MS)
- Xevo G2 QTof
- Xevo QTof MS
- Xevo TQ MS
- Xevo TQ-S
- ACQUITY TQD
THE LAZWELL PLATES
They are compatible with the robots of preparation which makes it possible to automate the preparation of your plates LAZWELL.
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