Get training to the new generation of Capillary Electrophoresis

WynSep organise training sessions to capillary electrophoresis in its location in Labège (31 – France) or directly on your location. The training is achieved by Cédric Sarazin, PhD from Pierre and Marie Curie University from Paris, specialized in analytical chemistry and former lecturer.
Duration: 1, 2 or 3 days. Modular programme depending on your needs and your requests

Place: Labège or your location

Relevant public: engineers, researchers, technicians, pharmacists

Aim of the training: introduce the fundamental principles of capillary electrophoresis and also the instrumentation and the main applications liked to this technique. (the objectives can be redefined according the requests)

THEORETICAL CONTENTS

  • Basic principles and generalities on electrokinetic techniques: electrophoretic migration, electroosmotic flow, detection mode (UV-Vis, C4D, LIF, mass spectrometry), injection mode, quantitative analysis, separation performance
  • Different modes of separation: capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC), chiral separation, capillary gel electrophoresis (CGE), capillary isoelectric focusing

DEMONSTRATIONS

  • capillary zone electrophoresis – le fundamental values: influence of the electrolyte pH, of the ionic strength, of the temperature, of the voltage. Calculation of the efficiencies, of the mobilities, separation optimisation
  • Inorganic anion and cation analysis: utilisation of a zero-flow coated capillary, initiation to conductimetry detection
  • Comparison of UV and C4D detection modes: example of organic acids in wine. Strengths and weaknesses of the both detection modes
  • Initiation of Laser Induced Fluorescence (LIF) detection
  • Separation of neutral compounds by MEKC: influence of surfactant concentration and of pH and analysis of the separation performance
  • Chiral separation: optimisation of a chiral separation of optical isomers with electrolytes containing cyclodextrins

THEY HAVE FOLLOWED THE WYNSEP TRAININGS

Training very interesting and complete. Our talker was able to respond to our particular needs. Mr Machin

Training very interesting and complete. Our talker was able to respond to our particular needs. Mr Machin

Training very interesting and complete. Our talker was able to respond to our particular needs. Mr Machin

To participate or organise a training on new generation of capillary electrophoresis :

Dr Cédric Sarazin helps you to determine your needs