The method presents the determination of relative amounts of albumin and globulins in human serum using the Wyn-CE system. Identification and quantitation of the analysed proteins is obtained by direct UV absorbance detection at 200 nmWyn-CE. The identification and quantification of the analyzed proteins is obtained by direct UV detection at 200 nm.
L'avis de l'expert : Ideal for teaching in Biochemistry. Thanks to the specially well adaptive carousel, samples can be directly injected in an Eppendorf vial.
Réactifs et conditions d'analyse
Tampon : borate, pH 10.0
Capillaire : bare-fused silica, L = 30 cm, l = 22 cm, ID = 50 µm
Injection : Hydrodynamic, 50 mbar, 8 s
Tension : +10 kV
Détection : UV 200 nm, direct
Température : 20 °C