Chiral separation of enantiomers of isoprenaline (with the University of Grenoble)

The isoprenaline racemate is a catecholamine used for the treatment of bradycardia and cardiac arrest. A chiral separation of the two forms is mandatory for the quantification of each enantiomer. In this application note, the Wyn-CE system coupled to non-contact conductivity detection was used for the separation of the two forms of isoprenaline. The effects of pH and Cyclodextrin concentration are described.

L'avis de l'expert : Capillary electrophoresis is a technique of choice for performing chiral analyzes.

Réactifs et conditions d'analyse

Tampon : Phosphate + HP-β-CD, pH variable
Capillaire : virgin bubble silica, L = 64.5 cm, l = 56 cm, ID = 50 μm Injection: hydrodynamic, 50 mbar, 4 s
Injection : hydrodynamic, 50 mbar, 4 s
Tension : +30 kV
Détection : UV, 200 nm
Température : 35 °C


Other applications Pharmaceutical quality control